Lamin A/C antibody (AA 400-450)

Catalog No. ABIN7455219

This Rabbit Polyclonal antibody specifically detects Lamin A/C in WB, IP, IF/ICC and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for Lamin A/C antibody (AA 400-450) (ABIN7455219)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Lamin A/C Antibody

Binding Specificity: AA 400-450

Purpose: Rabbit anti-Lamin-A Antibody, Affinity Purified

Predicted Reactivity: Rat,Pig

Purification: Affinity Purified

Immunogen: between AA 400 and 450

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:50 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin-A

Background: Background: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin A/C plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics [taken from the Universal Protein Resource (UniProt) P02545].

Gene ID: 4000

NCBI Accession: NP_733821

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus